Epitope Tag Antibodies

No. Size Price
M20020S 50ul ¥500
M20020M 100ul ¥900
M20020L 200ul ¥1600

Product Name

His-Tag mAb (HRP Conjugated)

Description

Isotype

Mouse IgG1

Molecular Weight

Source

This Abmart monoclonal antibody is produced by immunizing mice with a 6× His synthetic peptide (KLH-coupled).

Specifity

His-Tag mAb (HRP Conjugated ) detects over-expressed or recombinant proteins containing the His epitope tag.

Aliases

Storage

Store at -20°C. Stable for one year from the date of shipment.

Secondary Antibody (Important)

Application Tested

WB 1: 5000

Reactivity

All

Application Data

WB WB

Western blot analysis of his-tagged fusion protein.
Each lane was loaded with 5ng of his-tagged fusion protein.
Lane 1-6: 1: 0.5k, 1: 1k, 1: 2k, 1: 5k, 1: 10k, 1: 20k

IF

IF

WB WB

IHC IHC

Applications Key

Reactivity Key

AbMATE-2A8  A positive control for using

  • 1)
  • 2)

Failure to see above result suggests that your Western experiment system is NOT working, and likely your own Western experiment will not work with .  If you see the expected band and the dot ( is working), but your own experiment membrane is not what you expected, please look for other reasons (for example, your protein sample preparation) rather than Western system itself.

Please contact Abmart’s technical support team by phone (+86-21-64048322) or email (support@abmart.com.cn) for further questions.

AbMATE- can be stored at 4°C for 6 months.

(The AbMATE- should be submerged in methanol for 5 minutes before using).

Mate
Membrane before experiment

Mate
Film after experiment

Companion Products


For in vitro research use only and not intended for use in humans or animals