|
No.
|
Size
|
Price
|
|
P30008
|
100 μl
|
￥900
|
Product Name
GAPDH Antibody
Source
This Abmart polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to carboxy-terminal residues of human GAPDH.
Specifity
GAPDH Antibody detects endogenous levels of total GAPDH protein.
Storage
Store the product at -20°C. Stable for one year from the date of shipment.
Secondary Antibody (Important)
Use an anti-RABBIT secondary antibody to detect the antibody.
Application Tested
Western blotting 1:1000
Application Data
|
|
|
Western blot analysis of Hela whole cell lysate using GAPDH Antibody. The dilutions are 1:1000 (lane1), 1:2000 (lane2), 1:5000 (lane3). Each lane was loaded with 20 µg of Hela cell lysate.
|
Western blot analysis of lysates from various cell lines using GAPDH Antibody (1:1000). Each lane was loaded with 20 µg whole cell lysate. Lane 1: HeLa; Lane 2: 293T; Lane 3: NIH/3T3; Lane 4: COS7.
|
AbMATE-2A8 A positive control for using
|
Failure to see above result suggests that your Western experiment system is NOT
working, and likely your own Western experiment will not work with
. If you see the expected band and the dot (
is working), but your own experiment membrane is not what you expected, please look
for other reasons (for example, your protein sample preparation) rather than Western
system itself.
Please contact Abmart’s technical support team by phone (+86-21-64048322) or email
(support@abmart.com.cn) for further questions.
AbMATE-
can be stored at 4°C for 6 months.
(The AbMATE-
should be submerged in methanol for 5 minutes before using).
|
Membrane before experiment
Film after experiment
|
Companion Products
For in vitro research use only and not intended for use in humans or animals